08:30 – 09:00

Registration

09:00 – 09:10

Welcome remarks from the organizing team

Session 1

Time

Speaker

Title

09:10 – 09:40

Susana M. Cerritelli

Role of RNase H1 in the nucleus: Past and present insights.

Susana M. Cerritelli, Kiran Sakhuja, Caitlin L. Darling, Stella R Hartono, James Iben, Frédéric Chédin and Robert J. Crouch.

09:40 – 10:05

Frédéric Chédin

Manipulating RNase H1 levels in murine B cells dramatically affects mitochondrial genome maintenance but shows little impact on nuclear R-loop distributions.

Kiran Sakhuja, Lionel A Sanz, Stella R Hartono, Frédéric Chédin, Susana Cerritelli, Robert Crouch.

10:05 – 10:30

Sheetal Uppal

RNase H1 contributes to alternative splicing regulation.

Anusree Dey, Rituparna Das, Cu Nguyen, Chen Qingrongc, Daoud Meerzamanc, Sheetal Uppal.

10:30 – 11:00

Coffee break

11:00 – 11:25

Hai Dang Nguyen

RNase H1 phosphorylation at Ser76 promotes RPA interaction to safeguard genome integrity from R-loop-associated instability.

Victor M. Corral, Wannasiri Chiraphapphaiboon, Niraja Soman, Kali McDonough, Hannah Terry, and Hai Dang Nguyen.

Time

Speaker

Title

11:25 – 11:50

Petra Beli

Regulation of human RNase H1 by phosphorylation.

Ivan Mikicic, Magdalena Schachtl-Rieß, Justus Gräf, Christian Blum, Eric Schmidt, Katharina Mayr, Thorsten Mosler, Petra Beli.

11:50 – 12:15

Marcin Nowotny

Mechanism of the RNase H domain of reverse transcriptases.

Marcin Nowotny.

12:15 – 14:00

Lunch

Session 2

14:00 – 14:25

Robert J Crouch

Chaos leading to survival after loss of RNase HI and RecBCD in E. coli.

James Iben, Joshua Babiarz, Andrea Mota, Susana M. Cerritelli, Kiran Sakhuja, Marissa Valencia, Don Ennis, Ashley Bushy, Mitshiro Itaya, Shigenori Kanaya, and Robert J Crouch.

14:25 – 14:50

Andrei Kuzminov

UV-induced Replication-Transcription Conflicts.

Elena A. Kouzminova, Glen E. Cronan and Andrei Kuzminov.

14:50 – 16:15

Coffee break and poster session

16:15 – 16:40

Carolin Wagner

RNase H1 Prevents Transcription Replication Conflicts in the Absence of Sen1 in S. cerevisiae.

Carolin Wagner, Fabio Bento, Matteo Longaretti, Sophia Sergi, Arianna Lockhart, Ronald Wong, Vanessa Pires, Helle Ulrich, Brian Luke.

16:40 – 17:05

Vandit Shah

Investigate the potential role of RNase H1 in the development of chronic kidney disease.

Vandit Shah, Emilia Kieckhöfer, Gisela Slaats, Thomas Benzing, Bernhard Schermer.

17:05 – 17:30

Natalia Gromak

Toxic R-loops in Repeat Expansion Diseases.

Chiara Beghe, Michael Tellier, Rob Torrance, Helena Becker, Michael Flowerc, Shona Murphy, Sarah J Tabrizi, Natalia Gromak.

18:00

Dinner and get-together

Session 3

Time

Speaker

Title

09:00 – 09:30

Jessica Williams

High fidelity DNA ligation is critical for preventing genome instability.

Jessica S. Williams, Percy T. Tumbale, Thomas J. Jurkiw, Mercedes E. Arana, Julian A. Rana, Patrick J. O’Brien, Thomas A. Kunkel and R. Scott Williams.

09:30 – 09:55

Percy P. Tumbale

Molecular basis for RNA discrimination by human DNA ligase 1.

Percy P. Tumbale, Thomas J. Jurkiw, Juno M. Krahn, Lars C. Pedersen, Jessica S. Williams, Tom A. Kunkel, Patrick J. O'Brien, and R. Scott Williams.

09:55 – 10:20

Andrei Chabes

Impact of dNTP/rNTP Ratios on Ribonucleotide Incorporation into mtDNA.

Paulina H. Wanrooij, Phong Tran, Liam J. Thompson, Gustavo Carvalho, Sushma Sharma, Katrin Kreisel, Clara Navarrete, Anna-Lena Feldberg, Danielle L Watt , Anna Karin Nilsson, Martin K M Engqvist, Anders R Clausen, Andrei Chabes.

10:20 – 11:00

Coffee break

11:00 – 11:25

Paulina H. Wanrooij

Ribonucleotide removal pathways in mammalian mitochondria: the poor endoribonuclease activity of human mitochondrial topoisomerase protects against rNMP-dependent deletions.

Cyrielle P. J. Bader*, Erika Miyazaki-Kasho*, Josefin M. E. Forslund*, Malgorzata Wessels, and Paulina H. Wanrooij.

Time

Speaker

Title

11:25 – 11:40

Kristi L. Jensen

Genomic ribonucleotide metabolism contributes to replicative lifespan and is mis-regulated in aged yeast cells.

Kristi L. Jensen, Aleksandr Maliavko, Gilles Charvin, Brian Luke.

11:40 – 12:05

Diana Rios-Szwed

Synthetic lethality with RNase H2 and Ribonucleotide Excision Repair deficiency: new functions of EXO1 in mammalian DNA integrity.

Diana Rios-Szwed, Martin A. Reijns, Olga Murina, David Parry, Andrea Robertson, Paula Carroll, Simone Pelliciari, Tom Deegan, Andrew P. Jackson.

12:05 – 14:00

Lunch

Session 4

14:00 – 14:25

Kiyoshi Yasukawa

Leu606 of human DNA polymerase δ plays a role in discriminating deoxyribonucleotide and ribonucleotide.

Rin Ueno, Shu Ishibashi, Mako Kandabashi, Kentaro Fukata, Teisuke Takita, and Kiyoshi Yasukawa.

14:25 – 14:50

Maarten van den Ancker

Prediction of an evolutionary conserved complex between RNase H2 and Pol δ.

Maarten van den Ancker, Katy R. Astell, Simone Pelliciari, Ottavia Olson, Mihaly Badonyi, Joe Marsh, Tom D. Deegan, Andrew P. Jackson, Martin A.M. Reijns.

14:50 – 15:15

Pablo Huertas

R-loops and interferon contribute to hamper homologous recombination in Aicardi-Goutières Syndrome.

Andrea Moo, Amador Romero-Franco, Sonia Jimeno, and Pablo Huertas.

15:15 – 15:45

Coffee break

15:45 – 16:10

Akira Sassa

Uncovering distinct DNA damage responses associated with RNase H2 deficiency and its pathogenic mutation in human TK6 cells.

Kazuma Nakatani, Nao Terakoshi, Keigo Yamakita, Asuka Tachikawa, Hina Tanaka, Ayano Watanabe, Ken Takafuji, Yuiko Mayuzumi, Yui Yoshimoto, Takayuki Fukuda, Kaoru Sugasawa, Kiyoe Ura, and Akira Sassa.

16:10 – 16:35

Laura Hartleb

Initiation of DNA replication in Trypanosoma brucei is regulated by interaction of the RNase H2 complex with DOT1 histone methyltransferases.

Laura Hartleb, Victoria Frisbie, Hideharu Hashimoto, Janna Kiselar, Falk Butter, Erik W. Debler and Christian J. Janzen.

16:35 – 17:00

Erik W. Debler

Non-Canonical mechanisms of RNase H2 recruitment to PCNA.

Hideharu Hashimoto, Nancy Meyer, Janna Kiselar, Craig Yoshioka, and Erik W. Debler.

17:30 – 19:00

Social program

19:00 - …

Dinner and get-together

Session 5

Time

Speaker

Title

09:00 – 09:25

Philippe Pasero

Postreplicative RNA:DNA hybrids as a source of replication stress and inflammation.

Hervé Técher, Jonathan Heuzé, Bayan Chami, Antoine Barthe, Jérôme Poli, Armelle Lengronne, Yea-Lih Lin and Philippe Pasero.

09:25 – 09:50

Aziz El Hage

Genome-wide mapping of R-loops in cells deficient for the RNA exosome reveals enrichment of hybrids at sites of non-coding RNAs.

Aziz El Hage, Susana M. Cerritelli, Hywel-Dunn Davies, Shaun Webb, Pin Tong, Robert J. Crouch and David Tollervey.

09:50 – 10:15

Alex Bishop

Not all R-loops are equal.

Henry Miller, Nick Bassani, Liesl Lawrence, Pramiti Mukhopadhyay and Alex Bishop.

10:15 – 10:50

Coffee break

Time

Speaker

Title

10:50 – 11:15

Zicheng Yu

A machine-learning-based tool to detect DNA-embedded ribonucleotides from long-read nanopore sequencing.

Zicheng Yu, Thomas Williams, Zihao Chen, Craig Anderson, Martin Taylor, Andrew Jackson, and Martin Reijns.

11:15 – 11:40

Mo Sun

rNMPID: a database for ribonucleoside MonoPhosphates in DNA.

Mo Sun, Jingcheng Yang, Zihan Ran, Taewhan Yang, Deepali L. Kundnani, Francesca Storici, and Penghao Xu.

11:40 – 11:50

Closing remarks from the organizing team

12:20 – 14:00

Lunch and goodbye

14:00 – 15:00

Technical Talk with Alex Bishop

(open to everyone)

Focus of the discussion: R-loop database RLBase, and peculiarities of R-loop mapping techniques.